Journal: Cancer research

Article Title: Targeting brain-adaptive cancer stem cells prohibits brain metastatic colonization of triple-negative breast cancer

doi: 10.1158/0008-5472.CAN-17-2994

Figure Lengend Snippet: A. Oncomine database analysis of PCDH7 expression in normal human tissue (Neurogenetics 2006 7:67–80). B. Western Blot analysis of PCDH7 in independent brain metastasis-derived tumorspheres (TS1 and TS2) and corresponding brain-seeking (Br) cell lines. C. Protein expression of PCDH7 in TNBC patient brain metastasis tumorspheres (BM-TS1 and 2) and various cell models. MCF7, SUM159, SKBR3: human breast cancer cell lines; BoM1833: MB231 bone-seeking cell line; LM4175: MB231 lung seeking cell line; NMA: primary normal mouse astrocytes; NHA: normal human astrocytes; HBVEC: human brain microvascular endothelial cells. D. Receiver Operating Characteristic (ROC) curve for PCDH7 expression in primary breast tumor samples of brain metastatic patients using the combined 368 microarray data (MSK-82 and EMC-286 cohort). E. Kaplan–Meier curves showing the brain metastasis-free survival of patients with positive or negative PCDH7 expression in the combined cohort of 368 breast cancer patients, P=1.21×10−5 determined by log rank test. F. Representative PCDH7 immunohistochemistry staining of matched patient tissue sections of brain metastasis, lung metastasis and primary breast tumors. Scale bar: 20µm.

Article Snippet: Normal human astrocytes and human brain microvascular endothelial cells were purchased from Lonza Group Ltd (Allendale, NJ).

Techniques: Expressing, Western Blot, Derivative Assay, Microarray, Immunohistochemistry, Staining

Journal: Molecular Pharmacology

Article Title: A Dietary Agonist of Transient Receptor Potential Cation Channel V3 Elicits Endothelium-Dependent Vasodilation S⃞

doi: 10.1124/mol.109.060715

Figure Lengend Snippet: Carvacrol stimulates TRPV3 cation currents in cerebral artery endothelial cells A, representative whole-cell currents recorded from human microvascular cerebral artery endothelial cells during voltage ramps from −100 to +100 mV. Current density is shown for cells under baseline conditions (B) and after first (1), second (2), and third (3) application of carvacrol (100 μM). B, summary data demonstrating sensitization of the carvacrol-induced current (n = 6). C, summary data showing the effects of the TRPA1 antagonist HC-030031 (HC, 3 μM) and the TRPV1–4 blocker RuR (10 μM) on carvacrol-induced currents recorded from cerebral artery endothelial cells; n = 5 for HC-030031, n = 4 for ruthenium red. Current magnitude was normalized to peak carvacrol-induced currents. *, P ≤ 0.05 versus control (C).

Article Snippet: Carvacrol-activated currents were recorded from human cerebral artery microvascular endothelial cells (Cell Systems Corporation, Kirkland, WA).

Techniques: Control

Journal: Stem Cell Reviews and Reports

Article Title: Outgrowth Endothelial Cell Conditioned Medium Negates TNF-α-Evoked Cerebral Barrier Damage: A Reverse Translational Research to Explore Mechanisms

doi: 10.1007/s12015-022-10439-4

Figure Lengend Snippet: Schematic diagram of an in vitro model of human BBB and the effect of OEC-CM on BBB integrity and function and actin cytoskeleton organization in HBMECs and OECs. (A) In vitro models of human BBB consisting of astrocytes, pericytes, and HBMECs alone or mixed with OECs. (B, C) TNF-α significantly disrupted BBB integrity and function, as shown by decreases in TEER and concomitant increases in paracellular flux of sodium fluorescein, which were prevented by OEC-CM treatment. (D) Co-treatment with OEC-CM prevented the effects of TNF-α on cytoskeletal reorganization in HBMECs and OECs and decreased stress fiber formation (white arrows). (E) Quantification of stress fiber formation in both cells. Scale bar: 25 μm. * P < 0.05 versus BBB formed by HBMECs or control, # P < 0.05 versus BBB formed by HBMECs exposed to TNF-α, † P < 0.05 versus BBB formed by HBMECs exposed to TNF-α and OEC-CM, φ P < 0.05 versus BBB formed by HBMECs and OECs, ψ P < 0.05 versus BBB formed by HBMECs and OECs exposed to TNF-α (one-way ANOVA followed by Tukey's post-hoc analysis). BBB, blood–brain barrier; HBMECs, human brain microvascular endothelial cells; OEC-CM, outgrowth endothelial cell-derived conditioned medium; OECs, outgrowth endothelial cells; TNF-α, tumor necrosis factor-α

Article Snippet: Human brain microvascular endothelial cells (HBMECs), pericytes, and astrocytes were purchased from TCS CellWorks Ltd. (Buckingham, UK) and cultured at 37 °C in a humidified atmosphere (75% N 2 , 20% O 2 , 5% CO 2 ) with their respective media (Sciencell Research Laboratories, San Diego, USA).

Techniques: In Vitro, Control, Derivative Assay

Journal: Stem Cell Reviews and Reports

Article Title: Outgrowth Endothelial Cell Conditioned Medium Negates TNF-α-Evoked Cerebral Barrier Damage: A Reverse Translational Research to Explore Mechanisms

doi: 10.1007/s12015-022-10439-4

Figure Lengend Snippet: The effect of OEC-CM on HBMEC and OEC functional characteristics and analysis of angiogenesis-related proteins in HBMEC and OEC secretomes and OEC-CM. (A, B) OEC-CM accelerated wound closure in both HBMEC and OECs. (C-E) OEC-CM negated the impact of TNF-α on HBMEC and OEC tubule network. (F, G) Treatments with OEC-CM neutralized the inhibitory effect of TNF-α on HBMEC and OEC adhesion to fibronectin, an extracellular matrix protein. (H, I) Proteome profiling of OEC-CM along with HBMEC and OEC secretomes revealed significant variations in various pro- and anti-angiogenic factors e.g. endothelin-1, MCP-1 and endostatin in OEC-CM. Scale bars = 100 μm. * P < 0.05 versus control, # P < 0.05 versus TNF-α (one-way ANOVA followed by Tukey's post-hoc analysis). HBMECs, human brain microvascular endothelial cells; IL-8, interleukin-8; MCP-1, monocyte chemoattractant protein-1; OEC-CM, outgrowth endothelial cell-derived conditioned medium; OECs, outgrowth endothelial cells; TIMP-1, tissue inhibitors of metalloproteinase-1; TNF-α, tumor necrosis factor-α; uPA, urokinase plasminogen activator

Article Snippet: Human brain microvascular endothelial cells (HBMECs), pericytes, and astrocytes were purchased from TCS CellWorks Ltd. (Buckingham, UK) and cultured at 37 °C in a humidified atmosphere (75% N 2 , 20% O 2 , 5% CO 2 ) with their respective media (Sciencell Research Laboratories, San Diego, USA).

Techniques: Functional Assay, Control, Derivative Assay